Simmons Citrate Agar

 

Use: Simmons Citrate Agar is an agar medium used as a differential medium for the identification of enteric bacteria, using citrate as the sole source of carbon.

Description: Koserl developed a fluid medium to determine citrate utilization. Simmon further modified the formula by adding 1.5% agar and bromthymol blue as an indicator. The medium can be used to distinguish between Escherichia coli and Enterobacter aerogenes and to assist in differentiating members of the Salmonella group. Organisms able to utilize the citrate grow well on the surface of the medium and alkalinize the medium, changing it from green to blue. If the organism cannot utilize the citrate, poor or no growth is shown, and, the medium remains unchanged.

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