Orange Serum Agar

 

Use: Medium used for the cultivation of aciduric microorganisms, particularly those associated with the spoilage of citrus products.

Description: The low pH of fruit juices makes citrus fruit products susceptible to spoilage by yeasts, molds, and the bacteria Lactobacillus and Leuconostoc. In the 1950’s, Hays investigated spoilage in frozen concentrated orange juice. He found that an agar medium containing orange serum (juice) was superior to Lindegren Agar in isolation the microorganisms responsible for spoilage, causing a buttermilk off-odor. In a later comparative study, Murdock, Folinazzo, and Troy found Orange Serum Agar, pH 5.4 to be a suitable medium for growing Leuconostoc, Lactobacillus, and yeasts.

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