Bismuth Sulfite Agar

 

Use: Highly selective agar medium for the isolation of Salmonella, particularly Salmonella typhi from clinical and environmental specimens.

Description: In 1926 Wilson and Blair used Bismuth and Sodium Sulfite in a culture formulation for the isolation of typhoid-paratyphoid groups of bacteria. Further modifications of the formula were described by Hajna and Damon. The current recommended formulation is found in the USP. Bismuth and brililant green dye acts as inhibitors to most enteric bacteria and Gram positive bacteria. Salmonella typhi produces black colonies surrounded by a metallic sheen. Other Salmonella produce green or black colonies. Some Shigella sp. may grow on this medium.

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